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LearnPA5-18239
antibody from Invitrogen Antibodies
Targeting: KPNA4
IPOA3, MGC12217, MGC26703, QIP1, SRP3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-18239 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KPNA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine and rat based on sequence homology.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Disease-specific alteration of karyopherin-α subtype establishes feed-forward oncogenic signaling in head and neck squamous cell carcinoma.
Hazawa M, Sakai K, Kobayashi A, Yoshino H, Iga Y, Iwashima Y, Lim KS, Chih-Cheng Voon D, Jiang YY, Horike SI, Lin DC, Wong RW
Oncogene 2020 Mar;39(10):2212-2223
Oncogene 2020 Mar;39(10):2212-2223
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Testes lysate using Product # PA5-18239 at a concentration of 0.5 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KPNA4 by a KPNA4 monoclonal antibody (Product # PA5-18239) at a concentration of 1 µg/mL. Human Testes (A), Human Ovary (B), Mouse Testes (C), Rat Testes (D) and Rat Ovary (E) lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded of Human Small Intestine using Product # PA5-18239 at a concentration of 5 µg/mL. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded of Human Testis using Product # PA5-18239 at a concentration of 3 µg/mL. The tissue was processed by microwaved antigen retrieval with citrate buffer pH 6 and stained with HRP.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 KPNA4 prevent epidermal differentiation in HNSCCs. Epidermal-differentiation genes were analyzed by qRT-PCR ( a ) and western blot analysis ( b ) in HNSCCs cells. qRT-PCR data represent means +- SD ( n = 3). c The heat map showing mutual exclusivity between KPNA4 expression and differentiation-related genes. Samples were divided according to mRNA expression levels [mRNA expression z-Scores (RNA-Seq V2 RSEM) >mean +2.0SD] from the TCGA cohorts. P values are based on the fisher exact test. Epidermal-differentiation genes and KPNA4 were analyzed by qRT-PCR ( d ) and western blot analysis ( e , left) and quantification of KPNA4 proteins levels ( e , right) in HaCaT cells. Data represent means (qRT-PCR, n = 3) or means +- SD (Western Blot, n = 3). f , g Expression profiles of KPNA4 and IVL in normal skin tissue ( f ). Bar = 30 mum. Fluorescent intensity was quantified ( g ). h , i Three-dimensional reflective index tomographic analysis ( h ) of HaCaT and HNSCC cells. MIP means maximum intensity projection. i Quantif i cation of granulated particles were analyzed using TomoStudio. The two-tailed Student's t test was used to analyze the potential statistical difference between two groups. * p < 0.05.
