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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP54344PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP54344PU-N, RRID:AB_11148728
- Product name
- anti Thiamine pyrophosphokinase 1 (TPK1) (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the N-terminal region of Human TPK1
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of TPK1 Antibody (N-term) (AP54344PU-N) in mouse spleen tissue lysates (35 µg/lane). TPK1 (arrow) was detected using the purified Pab.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of TPK1 (arrow) using rabbit polyclonal TPK1 Antibody (N-term) (AP54344PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TPK1 gene.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human testis tissue reacted with TPK1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
