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- Product number
- GTX123679 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX123679, RRID:AB_11171013
- Product name
- Caspase 7 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE GTX123679 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 p11 subunit antibody (GTX123679) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Caspase 7 p11 subunit knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 p11 subunit antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Caspase 7 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Caspase 7 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) Jurakt whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 7 antibody (GTX123679) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and Caspase 7 knockout (KO) HeLa cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 7 p11 subunit antibody (GTX123679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using Caspase 7 p11 subunit(GTX123679) antibody at 1:500 dilution.
