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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- MAB6243 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6243, RRID:AB_10549920
- Product name
- CASP7 monoclonal antibody, clone 25B881.1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant CASP7.
- Isotype
- IgG
- Antibody clone number
- 25B881.1
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Caspases: the executioners of apoptosis.
Mch3, a novel human apoptotic cysteine protease highly related to CPP32.
Cohen GM
The Biochemical journal 1997 Aug 15;326 ( Pt 1):1-16
The Biochemical journal 1997 Aug 15;326 ( Pt 1):1-16
Mch3, a novel human apoptotic cysteine protease highly related to CPP32.
Fernandes-Alnemri T, Takahashi A, Armstrong R, Krebs J, Fritz L, Tomaselli KJ, Wang L, Yu Z, Croce CM, Salveson G
Cancer research 1995 Dec 15;55(24):6045-52
Cancer research 1995 Dec 15;55(24):6045-52
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CASP7 in Jurkat cells using CASP7 monoclonal antibody, clone 25B881.1 (Cat #MAB6243) at 1 ug/mL. Cells were treated with 2 uM staurosporine for different time periods. CASP7 activation is detected in western blots by the presence of CASP7 cleavage fragments. The antibody detected both pro (full-length) and active (cleaved) CASP7, depending on the treatment time points. A basal level of endogenous intermediate CASP7 can be see in untreated Jurkat cells.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CASP7 in A) human brain, B) mouse brain and C) rat brain lysate using CASP7 monoclonal antibody, clone 25B881.1 (Cat #MAB6243) at 1 ug/mL .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CASP7 in formalin-fixed, paraffin-embedded human cervix tumor tissue using an isotype control (top left) and CASP7 monoclonal antibody, clone 25B881.1 (Cat #MAB6243) (bottom left, right) at 5 ug/mL .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
