Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF823 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Caspase-7 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Caspase-7 precursor and the small Caspase-7 subunit that is generated during proteolytic activation.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The B30.2 domain of pyrin, the familial Mediterranean fever protein, interacts directly with caspase-1 to modulate IL-1beta production.
Chae JJ, Wood G, Masters SL, Richard K, Park G, Smith BJ, Kastner DL
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9982-7
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 27;103(26):9982-7
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human and Mouse Caspase-7 Precursor and Small Subunit by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for for the indicated times and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti- Human/Mouse Caspase-7 Polyclonal Antibody (Catalog # AF823), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Caspase-7 precursor and small subunit at approximately 32 and 7 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.