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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [6]
- Other assay [1]
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Validation data
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- Product number
- 10294-2-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCM3/PDCD10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.32 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat skin tissue were subjected to SDS PAGE followed by western blot with 10294-2-AP (CCM3/PDCD10 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB and IF result of CCM3 antibody (courtesy of Dr. Shah, University of Muenster). IF note: E15 mouse brain cortex was PFA fixed, the sections were antigen retrieved with Na-citrate buffer (pH 6) and blocked with NGS and Triton X100. 1:150 dilution of CCM3 antibody was used with overnight incubation of sections at 4 degrees. Secondary anti-Rabbit conjugated to AF488 was used to detect the staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 10294-2-AP (CCM3/PDCD10 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CCM3/PDCD10 (IP:10294-2-AP, 3ug; Detection:10294-2-AP 1:300) with mouse skeletal muscle tissue lysate 7500ug.
