Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27096 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRIP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of FANCJ using 30 µg of HeLa lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a FANCJ polyclonal antibody (Product # PA5-27096) at a dilution of 1:1000.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot using BRIP1 Polyclonal Antibody (Product # PA5-27096). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRIP1 Polyclonal Antibody (Product # PA5-27096) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FANCJ showing staining in the cytoplasm of 293T cells. 293T cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min and stained using a FANCJ polyclonal antibody (Product # PA5-27096) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRIP1 Polyclonal Antibody detects BRIP1 protein at cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min. Green: BRIP1 protein stained by BRIP1 Polyclonal Antibody (Product # PA5-27096) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- BRIP1 Polyclonal Antibody detects BRIP1 protein at nucleus on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. BRIP1 Polyclonal Antibody (Product # PA5-27096) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.