Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA308823 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal antibody to DNA ligase 3 (ligase III, DNA, ATP-dependent)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody to DNA ligase 3 (ligase III, DNA, ATP-dependent)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- LIG3
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 1.01mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (TA308823) diluted by 1:1000.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- DNA ligase III antibody detects LIG3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LIG3 protein stained by DNA ligase III antibody (TA308823) diluted at 1:500. Blue: Hoechst
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using DNA ligase 3(TA308823) antibody at 1:100 dilution.
- Validation comment
- IHC
