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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-52333 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DNA Ligase III Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: CDPRHKDCLL REFRKLCAMV ADNPSYNTKT QIIQDFLRKG SAGDGFHGDV YLTVKLLLPG VIKTVYNLND KQIVKLFSRI FNCNPDDMAR DLEQGDVSET IRVFFEQSKS FPPAAKSLLT IQEVDEFLLR LSKLTKE Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 93%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNA Ligase III in human cell line A-549 using a DNA Ligase III Polyclonal Antibody (Product # PA5-52333).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNA Ligase III in mouse cell line NIH-3T3 and rat cell line NBT-II using a DNA Ligase III Polyclonal Antibody (Product # PA5-52333).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DNA Ligase III in human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a DNA Ligase III Polyclonal Antibody (Product # PA5-52333).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DNA Ligase III in human testis tissue shows strong nuclear positivity in cells in seminiferus ducts. Samples were probed using a DNA Ligase III Polyclonal Antibody (Product # PA5-52333).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DNA Ligase III in human cell line U-251 MG using a DNA Ligase III Polyclonal Antibody (Product # PA5-52333) shows localization to nucleoplasm.
