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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [2]
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- Product number
- NBP1-41190 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-41190, RRID:AB_2136243
- Product name
- Mouse Monoclonal DNA Ligase III Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody will recognize human DNA ligase III.
- Reactivity
- Human, Mouse, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Arsenicals affect base excision repair by several mechanisms.
Widespread dependence of backup NHEJ on growth state: ramifications for the use of DNA-PK inhibitors.
DNA polymerase beta-dependent long patch base excision repair in living cells.
Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Rational design of human DNA ligase inhibitors that target cellular DNA replication and repair.
Low levels of DNA ligases III and IV sufficient for effective NHEJ.
ATM mediates oxidative stress-induced dephosphorylation of DNA ligase IIIalpha.
Translocation of XRCC1 and DNA ligase IIIalpha from centrosomes to chromosomes in response to DNA damage in mitotic human cells.
DNA ligase III as a candidate component of backup pathways of nonhomologous end joining.
Physical and functional interaction between DNA ligase IIIalpha and poly(ADP-Ribose) polymerase 1 in DNA single-strand break repair.
Ebert F, Weiss A, Bültemeyer M, Hamann I, Hartwig A, Schwerdtle T
Mutation research 2011 Oct 1;715(1-2):32-41
Mutation research 2011 Oct 1;715(1-2):32-41
Widespread dependence of backup NHEJ on growth state: ramifications for the use of DNA-PK inhibitors.
Singh SK, Wu W, Zhang L, Klammer H, Wang M, Iliakis G
International journal of radiation oncology, biology, physics 2011 Feb 1;79(2):540-8
International journal of radiation oncology, biology, physics 2011 Feb 1;79(2):540-8
DNA polymerase beta-dependent long patch base excision repair in living cells.
Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, Wilson SH
DNA repair 2010 Feb 4;9(2):109-19
DNA repair 2010 Feb 4;9(2):109-19
Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Guo Z, Zheng L, Dai H, Zhou M, Xu H, Shen B
Nucleic acids research 2009 Jun;37(10):3431-41
Nucleic acids research 2009 Jun;37(10):3431-41
Rational design of human DNA ligase inhibitors that target cellular DNA replication and repair.
Chen X, Zhong S, Zhu X, Dziegielewska B, Ellenberger T, Wilson GM, MacKerell AD Jr, Tomkinson AE
Cancer research 2008 May 1;68(9):3169-77
Cancer research 2008 May 1;68(9):3169-77
Low levels of DNA ligases III and IV sufficient for effective NHEJ.
Windhofer F, Wu W, Iliakis G
Journal of cellular physiology 2007 Nov;213(2):475-83
Journal of cellular physiology 2007 Nov;213(2):475-83
ATM mediates oxidative stress-induced dephosphorylation of DNA ligase IIIalpha.
Dong Z, Tomkinson AE
Nucleic acids research 2006;34(20):5721-279
Nucleic acids research 2006;34(20):5721-279
Translocation of XRCC1 and DNA ligase IIIalpha from centrosomes to chromosomes in response to DNA damage in mitotic human cells.
Okano S, Lan L, Tomkinson AE, Yasui A
Nucleic acids research 2005;33(1):422-9
Nucleic acids research 2005;33(1):422-9
DNA ligase III as a candidate component of backup pathways of nonhomologous end joining.
Wang H, Rosidi B, Perrault R, Wang M, Zhang L, Windhofer F, Iliakis G
Cancer research 2005 May 15;65(10):4020-30
Cancer research 2005 May 15;65(10):4020-30
Physical and functional interaction between DNA ligase IIIalpha and poly(ADP-Ribose) polymerase 1 in DNA single-strand break repair.
Leppard JB, Dong Z, Mackey ZB, Tomkinson AE
Molecular and cellular biology 2003 Aug;23(16):5919-27
Molecular and cellular biology 2003 Aug;23(16):5919-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - A. 293T whole cell lysate/extract. B. A431 whole cell lysate/extract. C. HeLa whole cell lysate/extract. D. HepG2 whole cell lysate/extract. DNA ligase III dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA Ligase III Antibody (1F3) [NBP1-41190] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
