600-401-480

antibody from Rockland Immunochemicals, Inc.

Targeting: PNKP PNK

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-480

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-480, RRID:AB_2162511

Product name

Anti-PNK (Polynucleotide Kinase) (RABBIT) Antibody - 600-401-480

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-PNK antibody shows detection of a 57 kDa band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10 ?g of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature. Signal was detected using standard techniques.

Validation comment

Western Blot