PAB21558

antibody from Abnova Corporation

Targeting: EFCAB3 FLJ25818

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21558

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21558, RRID:AB_10965723

Product name

EFCAB3 polyclonal antibody

Antibody type

Polyclonal

Antigen

Recombinant protein corresponding to amino acids of human EFCAB3.

Reactivity

Human

Host

Rabbit

Antigen sequence

LTKVPISHNKRDRDLPGSLQCQLQHKEKKLSASQM
AAFQDAYNFFYKDKTGCIDFHGLMCTVAKLGMNLT
KHDVYNELKCADIDRDGK


Isotype

IgG

Vial size

100 µl

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with EFCAB3 polyclonal antibody (Cat # PAB21558) at 1:250-1:500 dilution.

Validation comment

Western Blot (Transfected lysate)

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum with EFCAB3 polyclonal antibody (Cat # PAB21558) shows strong cytoplasmic and nuclear positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)