GTX17959

antibody from GeneTex

Targeting: KMT2A ALL-1, CXXC7, HRX, HTRX1, MLL, MLL1A, TRX1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX17959

Provider

GeneTex

Proper citation

GeneTex Cat#GTX17959, RRID:AB_422995

Product name

MLL antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Kmt2a cooperates with menin to suppress tumorigenesis in mouse pancreatic islets.

Lin W, Francis JM, Li H, Gao X, Pedamallu CS, Ernst P, Meyerson M
Cancer biology & therapy 2016 Dec;17(12):1274-1281

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

"Western Blot: MLL-1 Antibody (GTX17959) - Detection of Human and Mouse MLL1 by Western Blot. Samples: Nuclear extract (50 mcg for WT and KO; 10 mcg for EF, EN and EC) from 293T cells that were transfected with MLL1 c-terminal 180 kDa fragment (EC), 293T cells transfected with N-terminally truncated MLL1 fragment (EN; N300-tr), 293T cells transfected with MLL1 N-terminal 300 kDa fragment (EF), MLL1 +/+ mouse embryonic stem cells (WT), and MLL1 -/- embryonic stem cells (KO). Antibody: GTX17959 used at the indicated concentrations Detection: One second exposure with chemiluminescence"

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

"Western Blot: KMT2A/MLL Antibody (GTX17959) - Nuclear extract (NE; 15 and 50 mcg) and whole cell lysate (WCL; 15 and 50 mcg) from HeLa cells. Antibodies: Affinity purified rabbit anti-MLL1 antibody."

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow Cytometry: MLL-1 Antibody (GTX17959) - Flow cytometric detection of MLL1. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 mcg/ml anti-MLL1 GTX17959 in a 150 mcl reaction. Isotype control (black), anti- MLL1 (red).