PA3-011

antibody from Invitrogen Antibodies

Targeting: ERP29 C12orf8, ERp28, ERp31, PDI-DB, PDIA9

Antibody data

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Validation data

Product number: PA3-011 - Provider product page
Provider: Invitrogen Antibodies
Product name: ERP29 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: PA3-011 detects ERp29 protein in rat, mouse, hamster, canine, human, non-human primate, bovine and guinea pig samples.
Concentration: Conc. Not Determined

ERP29 protein structure - PA3-011 shown in red.

Merianda T, Twiss J
Neuroscience bulletin 2013 Aug;29(4):493-500

Sanderson TH, Deogracias MP, Nangia KK, Wang J, Krause GS, Kumar R
Neuroscience 2010 Sep 1;169(3):1307-14

Ogata T, Machida S, Oishi Y, Higuchi M, Muraoka I
Mechanisms of ageing and development 2009 May;130(5):328-36

Ethen CM, Reilly C, Feng X, Olsen TW, Ferrington DA
Investigative ophthalmology & visual science 2006 Jun;47(6):2280-90

Mkrtchian S, Fang C, Hellman U, Ingelman-Sundberg M
European journal of biochemistry 1998 Jan 15;251(1-2):304-13

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of ERp29 in HEK cell lysate using Product # PA3-011

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MDCK (Lane 1), NIH/3T3 (Lane 2), Hep G2 (Lane 3), A-549 (Lane 4), MCF-7 (Lane 5), A-431 (Lane 6) and HEK-293 (Lane 7). The blot was probed with Anti-ERp29 Rabbit polyclonal Antibody (Product # PA3-011, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 29 kDa band corresponding to ERp29 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence of ERp29 in rat thyrocytes using Product # PA3-011

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of ERp29 using anti-ERp29 polyclonal antibody (Product # PA3-011) shows staining in NS-1 Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of ERp29 using anti-ERp29 polyclonal antibody (Product # PA3-011) shows staining in p19 Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence of ERp29 in rat thyrocytes using Product # PA3-011

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of ERp29 using anti-ERp29 polyclonal antibody (Product # PA3-011) shows staining in HMVEC Cells.