Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00004350-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004350-M04, RRID:AB_2146157
- Product name
- Mouse Monoclonal MPG Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. MPG (1E10)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Chk2-dependent phosphorylation of XRCC1 in the DNA damage response promotes base excision repair.
Chou WC, Wang HC, Wong FH, Ding SL, Wu PE, Shieh SY, Shen CY
The EMBO journal 2008 Dec 3;27(23):3140-50
The EMBO journal 2008 Dec 3;27(23):3140-50
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MPG Antibody (1E10) [H00004350-M04] - Analysis of MPG expression in transfected 293T cell line by MPG monoclonal antibody (M04), clone 1E10. Lane 1: MPG transfected lysatE (32.2 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MPG Antibody (1E10) [H00004350-M04] - Analysis of MPG expression in Hela S3 NE (Cat # L013V3).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MPG Antibody (1E10) [H00004350-M04] - Detection limit for recombinant GST tagged MPG is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MPG Antibody (1E10) [H00004350-M04] - Analysis of monoclonal antibody to MPG on HeLa cell. Antibody concentration 40 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MPG Antibody (1E10) [H00004350-M04] - Analysis of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml