PA1-16507

antibody from Invitrogen Antibodies

Targeting: NUDT1 MTH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-16507

Provider

Invitrogen Antibodies

Product name

MTH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

The target sequence has 91% sequence homology with mouse and rat.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot detection of MTH1 in NT2 cells and 833K54CP9 cells using Product # PA1-16507.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate. Samples were incubated in MTH1 polyclonal antibody (Product # PA1-16507).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MTH1 in 0.5 mg/mL A431 lysate. Samples were incubated in MTH1 polyclonal antibody (Product # PA1-16507). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of MTH1 in A431 cells fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. Samples were incubated in MTH1 polyclonal antibody (Product # PA1-16507) using a dilution of 1:200 dilution overnight at 4 °C followed by anti-rabbit DyLight 488 (Green) with a dilution of 1:500. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of MTH1 (Green) in Hela cells using Product # PA1-16507. Nuclei (Blue) are counterstained with Hoechst 33258.