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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-34871 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCL2L12 Recombinant Rabbit Monoclonal Antibody (JE51-66)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: MCF-7 cell lysates, PC-3M cell lysates, A431, SW620, human lung carcinoma tissue, human gastric carcinoma tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JE51-66
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCL2L12 in Positive control: Lane 1: MCF-7 cell lysates, Lane 2: PC-3M cell lysates. Samples were transferred to PVDF membrane, blocked with 5% BSA (1 hour), incubated with BCL2L12 monoclonal antibody (Product # MA5-34871), at a dilution of 1:500, followed by Goat Anti-Rabbit IgG-HRP (1 hour) with a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BCL2L12 in A431 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with BCL2L12 monoclonal antibody (Product # MA5-34871) at a dilution of 1:100 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BCL2L12 in SW620 cells (green). Samples were formalin fixed, permeabilized with 0.1% Triton X-100 in TBS (1 hour, room temperature) and blocked with 1% BSA (15 min, room temperature), incubated with BCL2L12 monoclonal antibody (Product # MA5-34871) at a dilution of 1:50 (1 hour, room temperature), and followed by Alexa Fluor 488 Goat anti-Rabbit IgG and DAPI (blue) with a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BCL2L12 in paraffin-embedded human lung carcinoma tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with BCL2L12 monoclonal antibody (Product # MA5-34871), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BCL2L12 in paraffin-embedded human gastric carcinoma tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with BCL2L12 monoclonal antibody (Product # MA5-34871), and followed by HRP conjugated compact polymer system, DAB, counterstained with hematoxylin (mounted with DPX).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of BCL2L12 in A431 cells, unlabelled sample (control; cells without incubation with primary antibody; black). Samples were incubated with BCL2L12 monoclonal antibody (Product # MA5-34871) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG with a dilution of 1:1000 (30 min).
