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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [5]
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- Product number
- NB100-2251 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2251, RRID:AB_2171483
- Product name
- Rabbit Polyclonal PRMT1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references TbPRMT6 is a type I protein arginine methyltransferase that contributes to cytokinesis in Trypanosoma brucei.
Fisk JC, Zurita-Lopez C, Sayegh J, Tomasello DL, Clarke SG, Read LK
Eukaryotic cell 2010 Jun;9(6):866-77
Eukaryotic cell 2010 Jun;9(6):866-77
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PRMT1 Antibody [NB100-2251] - Detection of Human and Mouse PRMT1 on HeLa whole cell lysate using NB100-2251. PRMT1 was also immunoprecipitated using rabbit anti-PRMT1 antibody NB100-2250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PRMT1 Antibody [NB100-2251] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PRMT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PRMT1 Antibody [NB100-2251] - Detection of human PRMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer + 0.3% SDS, 1.5% sodium deoxycholate. Antibodies: Affinity purified rabbit anti-PRMT1 antibody NB100-2251 (lot NB100-2251-2) used for IP at 3 ug per reaction. PRMT1 was also immunoprecipitated by another rabbit anti-PRMT1 antibody. For blotting immunoprecipitated PRMT1, NB100-2251 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PRMT1 Antibody [NB100-2251] - Sample: FFPE sections of human small cell lung cancer (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- PRMT1 used at a dilution of 1:200 (1 ug/ml) and 1:1,000 (0.2 ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - FFPE section of human prostate adenocarcinoma. Antibody used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit antiPRMT1 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PRMT1 used at a dilution of 1:200 (1 ug/ml). Detection: DAB
