LS-C313073

antibody from LSBio

Targeting: ASIC3 ACCN3, DRASIC, TNaC1

Western blot (Enhanced validation)

Antibody Data

Product number

LS-C313073

Provider

LSBio

Product name

ACCN3 / ASIC3 Antibody (aa56-73) LS-C313073

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB of ACCN3 / ASIC3 antibody. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: U87 Cell Lysate. Lane 4: NEURO Cell Lysate. Lane 5: SMMC Cell Lysate.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of ASIC3 using anti-ASIC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Testis Tissue Lysate, Lane 3: U87 Cell Lysate, Lane 4: NEURO Cell Lysate, Lane 5: SMMC Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ASIC3 at approximately 69KD. The expected band size for ASIC3 is at 58KD.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of ASIC3 using anti-ASIC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Testis Tissue Lysate, Lane 3: U87 Cell Lysate, Lane 4: NEURO Cell Lysate, Lane 5: SMMC Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASIC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ASIC3 at approximately 69KD. The expected band size for ASIC3 is at 58KD.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB of ACCN3 / ASIC3 antibody. Lane 1: Rat Brain Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: U87 Cell Lysate. Lane 4: NEURO Cell Lysate. Lane 5: SMMC Cell Lysate.