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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-17166 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPA70 Monoclonal Antibody (4C4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17166 targets RPA1 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Non-human primate samples.
- Antibody clone number
- 4C4
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RPA1 using a RPA1 monoclonal antibody (Product # MA5-17166) against a human RPA1 (AA: 308-513) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), A-431 (Lane 3), K-562 (Lane 4), Hep G2 (Lane 5), HCT 116 (Lane 6), Panc-1 (Lane 7), U-2 OS (Lane 8) and COS-7 (Lane 9). The blot was probed with Anti- PCNA Polyclonal Antibody (Product # MA5-17166, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). A 70 kDa band corresponding to RPA70 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of RPA70 was achieved by transfecting HeLa cells with RPA70 specific siRNAs (Silencer® select Product # s12127, s12129). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the RPA70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with RPA70 Monoclonal Antibody (4C4) (Product # MA5-17166, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to RPA70.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RPA1 using a RPA1 monoclonal antibody (Product # MA5-17166) against a human RPA1 (AA: 308-513) recombinant protein.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using RPA1 monoclonal antibody (Product # MA5-17166) (Green). Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded colon cancer tissues using RPA1 monoclonal antibody (Product # MA5-17166) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded prostate tissues using RPA1 monoclonal antibody (Product # MA5-17166) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using RPA1 monoclonal antibody (Product # MA5-17166) (green) and negative control (red).
