Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-21976 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPA70 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse liver.
- Concentration
- 0.4 mg/mL
Submitted references Replication stress is a potent driver of functional decline in ageing haematopoietic stem cells.
Flach J, Bakker ST, Mohrin M, Conroy PC, Pietras EM, Reynaud D, Alvarez S, Diolaiti ME, Ugarte F, Forsberg EC, Le Beau MM, Stohr BA, Méndez J, Morrison CG, Passegué E
Nature 2014 Aug 14;512(7513):198-202
Nature 2014 Aug 14;512(7513):198-202
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), K-562 (Lane 3), Hep G2 (Lane 4), HCT 116 (Lane 5), Panc-1 (Lane 6) and U-2 OS (Lane 7). The blot was probed with Anti- PCNA Polyclonal Antibody (Product # MA5-17166, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). A 70 kDa band corresponding to RPA70 was observed across the cell lines tested with additional non specific band at 35 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of RPA70 was achieved by transfecting HeLa cells with RPA70 specific siRNAs (Silencer® select Product # s12127, s12129). Western blot analysis (Fig. a) was performed using modified whole cell extracts (1% SDS) from the RPA70 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with RPA70 Polyclonal Antibody (Product # PA5-21976, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to RPA70.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPA70 was performed by separating 50 µg of mouse liver lysates by 7.5% SDS PAGE. Proteins were transferred to a membrane and probed with a RPA70 Polyclonal Antibody (Product # PA5-21976) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPA70 antibody detects RPA70 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 antibody RPA70 Polyclonal Antibody (Product # PA5-21976) diluted by 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPA70 Polyclonal Antibody detects RPA70 protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RPA70 Polyclonal Antibody (Product # PA5-21976) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RPA 70 kDa subunit in paraformaldehyde-fixed HeLa cells using a RPA 70 kDa subunit polyclonal antibody (Product # PA5-21976) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit antibody (Product # PA5-21976) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RPA70 was performed in paraffin-embedded human lung papillary adenocarcinoma tissue using RPA70 Polyclonal Antibody (Product # PA5-21976) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit (Product # PA5-21976) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPA70 Polyclonal Antibody detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. RPA70 Polyclonal Antibody (Product # PA5-21976) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
