H00004595-M01
antibody from Novus Biologicals
Targeting: MUTYH
MYH
Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00004595-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004595-M01, RRID:AB_540648
- Product name
- Mouse Monoclonal MYH Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MUTYH - mutY homolog (E. coli)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Reduced expression of the DNA glycosylase gene MUTYH is associated with an increased number of somatic mutations via a reduction in the DNA repair capacity in prostate adenocarcinoma.
hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia.
Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia coli Strain.
Impaired 8-hydroxyguanine repair activity of MUTYH variant p.Arg109Trp found in a Japanese patient with early-onset colorectal cancer.
Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation.
Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells.
Human MutY homolog induces apoptosis in etoposide-treated HEK293 cells.
Reduced expression of MUTYH with suppressive activity against mutations caused by 8-hydroxyguanine is a novel predictor of a poor prognosis in human gastric cancer.
Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine.
Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis.
Shinmura K, Kato H, Kawanishi Y, Yoshimura K, Igarashi H, Goto M, Tao H, Inoue Y, Sugiyama T, Furuse H, Ozono S, Sugimura H
Molecular carcinogenesis 2017 Feb;56(2):781-788
Molecular carcinogenesis 2017 Feb;56(2):781-788
hMYH and hMTH1 cooperate for survival in mismatch repair defective T-cell acute lymphoblastic leukemia.
Eshtad S, Mavajian Z, Rudd SG, Visnes T, Boström J, Altun M, Helleday T
Oncogenesis 2016 Dec 5;5(12):e275
Oncogenesis 2016 Dec 5;5(12):e275
Functional Complementation Assay for 47 MUTYH Variants in a MutY-Disrupted Escherichia coli Strain.
Komine K, Shimodaira H, Takao M, Soeda H, Zhang X, Takahashi M, Ishioka C
Human mutation 2015 Jul;36(7):704-11
Human mutation 2015 Jul;36(7):704-11
Impaired 8-hydroxyguanine repair activity of MUTYH variant p.Arg109Trp found in a Japanese patient with early-onset colorectal cancer.
Shinmura K, Goto M, Tao H, Kato H, Suzuki R, Nakamura S, Matsuda T, Yin G, Morita M, Kono S, Sugimura H
Oxidative medicine and cellular longevity 2014;2014:617351
Oxidative medicine and cellular longevity 2014;2014:617351
Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation.
Agustina L, Hahm SH, Han SH, Tran AH, Chung JH, Park JH, Park JW, Han YS
BMC molecular biology 2014 Aug 15;15:17
BMC molecular biology 2014 Aug 15;15:17
Cancer-associated variants and a common polymorphism of MUTYH exhibit reduced repair of oxidative DNA damage using a GFP-based assay in mammalian cells.
Raetz AG, Xie Y, Kundu S, Brinkmeyer MK, Chang C, David SS
Carcinogenesis 2012 Nov;33(11):2301-9
Carcinogenesis 2012 Nov;33(11):2301-9
Human MutY homolog induces apoptosis in etoposide-treated HEK293 cells.
Hahm SH, Chung JH, Agustina L, Han SH, Yoon IS, Park JH, Kang LW, Park JW, Na JJ, Han YS
Oncology letters 2012 Dec;4(6):1203-1208
Oncology letters 2012 Dec;4(6):1203-1208
Reduced expression of MUTYH with suppressive activity against mutations caused by 8-hydroxyguanine is a novel predictor of a poor prognosis in human gastric cancer.
Shinmura K, Goto M, Suzuki M, Tao H, Yamada H, Igarashi H, Matsuura S, Maeda M, Konno H, Matsuda T, Sugimura H
The Journal of pathology 2011 Nov;225(3):414-23
The Journal of pathology 2011 Nov;225(3):414-23
Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine.
Suzuki T, Harashima H, Kamiya H
DNA repair 2010 May 4;9(5):542-50
DNA repair 2010 May 4;9(5):542-50
Immunohistochemistry is not an accurate first step towards the molecular diagnosis of MUTYH-associated polyposis.
van der Post RS, Kets CM, Ligtenberg MJ, van Krieken JH, Hoogerbrugge N
Virchows Archiv : an international journal of pathology 2009 Jan;454(1):25-9
Virchows Archiv : an international journal of pathology 2009 Jan;454(1):25-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi ( Cat # H00004595-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody (M01), clone 4D10 (Cat # H00004595-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody (M01), clone 4D10.Lane 1: MUTYH transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MYH Antibody (4D10) [H00004595-M01] - MUTYH monoclonal antibody (M01), clone 4D10 Analysis of MUTYH expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MYH Antibody (4D10) [H00004595-M01] - Detection limit for recombinant GST tagged MUTYH is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MYH Antibody (4D10) [H00004595-M01] - Analysis of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH MaxPab rabbit polyclonal antibody.