Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 679102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#679102, RRID:AB_2566076
- Product name
- Purified anti-CARD9
- Antibody type
- Monoclonal
- Antigen
- Human CARD9 peptide (LRKMQKGWRQGEEDRENT) conjugated to KLH.
- Description
- Caspase recruitment domain-containing protein 9 (CARD9) is a member of the CARD protein family, which is known to play regulatory roles in cell apoptosis. CARD9 is an adaptor protein that mediates the signaling downstream of C-type lectin receptors (CLRs), such as Dectin-1, Dectin-2, and Mincle. CARD9 also participates in the signaling downstream of the Retinoic acid-inducible gene I receptor (RIG-1) family. After stimulation of the receptors, CARD9 is phosphorylated and forms a trimolecular complex with B cell leukemia-lymphoma 10 (Bcl10) and mucosa-associated lymphoid tissue 1 (Malt1), which activates the canonical NF-κB pathway and leads to the induction of proimflammatory cytokines, including IL-6, IL-1β, and tumor necrosis factors-alpha (TNFs). CARD9 has been suggested to play an important role in antifungal host defense, as the CARD9 deficient mice are more susceptible to pathogenic fungi infections.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 13D9C60
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysates from THP-1 cells (lane 1), HeLa cells (lane 2), and RAW264.7 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal CARD9 (clone 13D9C60) antibody. Proteins were visualized using a HRP-conjugated anti-mouse-IgG secondary and chemiluminescence detection.
- Conjugate
- Unconjugated