AF714
antibody from Novus Biologicals
Targeting: GFRA1
GDNFR, GDNFRA, GFR-ALPHA-1, RET1L, RETL1, TRNR1
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- AF714 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal GFR alpha-1/GDNF R alpha-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human GFR alpha-1/GDNF R alpha-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat GFR alpha-1 is observed and less than 5% cross-reactivity with recombinant human (rh) GFR alpha-2, rhGFR alpha-3, and rhGFR alpha-4 is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references PLZF(pos)c-KIT(pos)-delineated A(1)-A(4)-differentiating spermatogonia by subset and stage detection upon Bouin fixation.
An Anti-GDNF Family Receptor Alpha 1 (GFRA1) Antibody-Drug Conjugate for the Treatment of Hormone Receptor-Positive Breast Cancer.
SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
Trophic factor modulation of cocaine- and amphetamine-regulated transcript peptide expression in explant cultured guinea-pig cardiac neurons.
Tang RL, Fan LQ
Asian journal of andrology 2019 May-Jun;21(3):309-318
Asian journal of andrology 2019 May-Jun;21(3):309-318
An Anti-GDNF Family Receptor Alpha 1 (GFRA1) Antibody-Drug Conjugate for the Treatment of Hormone Receptor-Positive Breast Cancer.
Bhakta S, Crocker LM, Chen Y, Hazen M, Schutten MM, Li D, Kuijl C, Ohri R, Zhong F, Poon KA, Go MAT, Cheng E, Piskol R, Firestein R, Fourie-O'Donohue A, Kozak KR, Raab H, Hongo JA, Sampath D, Dennis MS, Scheller RH, Polakis P, Junutula JR
Molecular cancer therapeutics 2018 Mar;17(3):638-649
Molecular cancer therapeutics 2018 Mar;17(3):638-649
SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP Jr
Development (Cambridge, England) 2007 Sep;134(18):3327-38
Development (Cambridge, England) 2007 Sep;134(18):3327-38
Trophic factor modulation of cocaine- and amphetamine-regulated transcript peptide expression in explant cultured guinea-pig cardiac neurons.
Girard BM, Young BA, Buttolph TR, Locknar SA, White SL, Parsons RL
Neuroscience 2006;139(4):1329-41
Neuroscience 2006;139(4):1329-41
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- GFR alpha-1/GDNF R alpha-1 in Human Spinal Cord. GFR alpha-1/GDNF R alpha-1 was detected in immersion fixed paraffin-embedded sections of human spinal cord using Goat Anti-Human GFR alpha-1/GDNF R alpha-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF714) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to ventral horn motoneurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.