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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-82270 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MDC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: REAVLKDHTK IRALVRAHSE KDQPPFGDSD DSVEADKSSP GIHLERSQAS TTVDINTQVE KEVPPGSAII HIKKHQVSVE GTNQTDVKAV GGPAKLLVVS LEEAWPLHGD CETDAEEGTS LTASVVADVR KSQLPAEGDA G
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Parvovirus nonstructural protein 2 interacts with chromatin-regulating cellular proteins.
Mattola S, Salokas K, Aho V, Mäntylä E, Salminen S, Hakanen S, Niskanen EA, Svirskaite J, Ihalainen TO, Airenne KJ, Kaikkonen-Määttä M, Parrish CR, Varjosalo M, Vihinen-Ranta M
PLoS pathogens 2022 Apr;18(4):e1010353
PLoS pathogens 2022 Apr;18(4):e1010353
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MDC1 in U-251 MG cells using a MDC1 polyclonal antibody (Product # PA5-82270). The analysis shows localization to nucleoplasm, nuclear bodies & focal adhesion sites.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MDC1 in human testis and liver tissues using a MDC1 polyclonal antibody (Product # PA5-82270). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MDC1 in human testis using a MDC1 polyclonal antibody (Product # PA5-82270). The analysis shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MDC1 in human liver using a MDC1 polyclonal antibody (Product # PA5-82270). The analysis shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 10.1371/journal.ppat.1010353.g008 Fig 8 Mutation of NS2 induce alteration in the amount and distribution of DDR proteins. Representative confocal images show the nuclear localization of NS1 (green), ( A ) gamma-H2AX (magenta), (B ) MDC1 (magenta) and DAPI staining (gray) in HeLa cells without transfection or at 24 hpt with wt, NS2 splice donor and acceptor mutants. Scale bars, 5 mum. (C ) Total fluorescence intensities of gamma-H2AX together with ( D ) its nuclear distribution as a function of increasing distance from the NE in nontransfected, wt and NS2 mutants transfected cells (n = 29). (E) Intensity line profiles of gamma-H2AX (purple) and NS1 (green) measured through the nucleoli. ( F ) Fluorescent intensities and ( G ) nuclear localization of MDC1 from the NE (n = 28). The error bars show the standard error of the mean. (H) Intensity line profiles of MDC1 (purple) and NS1 (green) measured through the nucleoli. Statistical significances were determined using Dunnett's multiple comparison test. The significance values shown are denoted as ** (p
