NBP2-12890

antibody from Novus Biologicals

Targeting: MDC1 Em:AB023051.5, KIAA0170, NFBD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NBP2-12890

Provider

Novus Biologicals

Product name

Mouse Monoclonal MDC1 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Detects approx 184kDa. This antibody recognizes MDC1 at and around the N-terminus.

Reactivity

Human, Mouse, Bovine

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - analysis of Mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Lane 1: MW ladder. Lane 2: Mouse Cortex. Lane 3: Mouse Cerebellum. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MDC1 Antibody (P2B11) [NBP2-12890] - analysis of Human 293Trap cell lysates showing detection of 184 kDa MDC1 protein using Mouse Anti-MDC1 Monoclonal Antibody, Clone P2B11 . Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MDC1 Monoclonal Antibody at 1:1000 for 2 hours RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 184 kDa.