HPA028954

antibody from Atlas Antibodies

Targeting: RAD54L hHR54, hRAD54, RAD54A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA028954

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA028954, RRID:AB_2672840

Product name

Anti-RAD54L

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

TLMWTLLRQSPECKPEIDKAVVVSPSSLVKNWYNE
VGKWLGGRIQPLAIDGGSKDEIDQKLEGFMNQRGA
RVSSPILIISYETFRLHVGVLQKGSVGLVICDEGH
RLKNSENQTYQALDSLNTSRRVLISGTPIQNDLLE
YFSLV


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and RAD54L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418575).

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.

Sample type

HUMAN