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Western blot
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- Antibody Data
- Antigen structure
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- Product number
- AP09254PU-N - Provider product page
- Provider
- OriGene
- Product name
- RAD54 (RAD54L) (1-17) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- RAD54 (RAD54L) (1-17) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- RAD54L
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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- OriGene (provider)
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- Experimental details
- Figure 1. Model for generation of supercoiling by hRad54 translocation along DNA. The hRad54 complex and plasmid DNA are indicated by the shaded oval and black line, respectively. (A) Movement of the hRad54 complex by tracking along the helical path of DNA is indicated by the arrows. When the complex is free to rotate around the DNA, no change in supercoiling will be induced in the plasmid DNA. (B) When the hRad54 complex tracks along the helix, while being prevented from rotating around the DNA, positive supercoils will arise ahead of the protein complex and negative supercoils behind it. These supercoils can freely distribute along the plasmid and therefore they will cancel each other out. (C) The interaction of two hRad54 complexes on a plasmid will divide the plasmid into two domains. Because the plasmid is singly nicked, one domain will contain a nick, whereas the other contains two covalently closed DNA strands. Depending on the position of the nick relative to the movement of the protein complex along the DNA, topoisomers containing either negative or positive supercoils will result after ligation of the nick.
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