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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA301478 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Antibody against Eg5
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Antibody against Eg5
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KIF11
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Cyclin A2 regulates symmetrical mitotic spindle formation and centrosome amplification in human colon cancer cells.
Li JA, Liu BC, Song Y, Chen X
American journal of translational research 2018;10(8):2669-2676
American journal of translational research 2018;10(8):2669-2676
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: Eg5 Antibody TA301478 - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY KIF11 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIF11.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Staining of HeLa cells fixed in 3.5% paraformaldehyde (1:5,000). Centrosome staining during interphase and spindle pole staining during mitosis.
- Validation comment
- IF
