Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG53992 - Provider product page
- Provider
- Arigo
- Product name
- anti-Eg5 antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human Eg5 protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4H3-1F12
- Vial size
- 100 µl
- Concentration
- Lot dependent
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: Eg5 in MCF7, 293T, Jurkat and HeLa cell lysates stained with Eg5 antibody [4H3-1F12] (ARG53992) (diluted in 1: 1000). Predicted band size: 130KDa. Observed band size: 130KDa.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa stained with Eg5 antibody [4H3-1F12] (ARG53992) (diluted in 1:200).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: Jurkat cell lysates immunoprecipitated and stained by Eg5 antibody [4H3-1F12] (ARG53992).
