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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP51185PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP51185PU-N, RRID:AB_11148383
- Product name
- anti DARS1 (N-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 161-190 amino acids from the N-terminal region of Human DARS.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of DARS (arrow) using DARS1 Antibody (N-term) Cat.-No AP51185PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of DARS1 Antibody (N-term) Cat.-No AP51185PU-NÂ in Jurkat cell line lysates (35ug/lane). This demonstrates the DARS antibody detected the DARS protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of DARS1 Antibody (N-term) Cat.-No AP51185PU-N with HepG2 cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human liver tissue stained with DARS1 Antibody (N-term) Cat.-No AP51185PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
