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- References [6]
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- ELISA [1]
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- Product number
- H00006009-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006009-M01, RRID:AB_1146837
- Product name
- Mouse Monoclonal Rheb Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RHEB - Ras homolog enriched in brain (2C11)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Ubiquitination of Rheb governs growth factor-induced mTORC1 activation.
The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.
RHEB1 insufficiency in aged male mice is associated with stress-induced seizures.
Identification of mechanically regulated phosphorylation sites on tuberin (TSC2) that control mechanistic target of rapamycin (mTOR) signaling.
p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb.
Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome.
Deng L, Chen L, Zhao L, Xu Y, Peng X, Wang X, Ding L, Jin J, Teng H, Wang Y, Pan W, Yu F, Liao L, Li L, Ge X, Wang P
Cell research 2019 Feb;29(2):136-150
Cell research 2019 Feb;29(2):136-150
The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.
Hoxhaj G, Hughes-Hallett J, Timson RC, Ilagan E, Yuan M, Asara JM, Ben-Sahra I, Manning BD
Cell reports 2017 Oct 31;21(5):1331-1346
Cell reports 2017 Oct 31;21(5):1331-1346
RHEB1 insufficiency in aged male mice is associated with stress-induced seizures.
Tian Q, Gromov P, Clement JH, Wang Y, Riemann M, Weih F, Sun XX, Dai MS, Fedorov LM
GeroScience 2017 Dec;39(5-6):557-570
GeroScience 2017 Dec;39(5-6):557-570
Identification of mechanically regulated phosphorylation sites on tuberin (TSC2) that control mechanistic target of rapamycin (mTOR) signaling.
Jacobs BL, McNally RM, Kim KJ, Blanco R, Privett RE, You JS, Hornberger TA
The Journal of biological chemistry 2017 Apr 28;292(17):6987-6997
The Journal of biological chemistry 2017 Apr 28;292(17):6987-6997
p53 Deletion or Hotspot Mutations Enhance mTORC1 Activity by Altering Lysosomal Dynamics of TSC2 and Rheb.
Agarwal S, Bell CM, Taylor SM, Moran RG
Molecular cancer research : MCR 2016 Jan;14(1):66-77
Molecular cancer research : MCR 2016 Jan;14(1):66-77
Spatial control of the TSC complex integrates insulin and nutrient regulation of mTORC1 at the lysosome.
Menon S, Dibble CC, Talbott G, Hoxhaj G, Valvezan AJ, Takahashi H, Cantley LC, Manning BD
Cell 2014 Feb 13;156(4):771-85
Cell 2014 Feb 13;156(4):771-85
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in NIH/3T3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rheb Antibody (2C11) [H00006009-M01] - RHEB monoclonal antibody (M01), clone 2C11. Analysis of RHEB expression in PC-12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Rheb Antibody (2C11) [H00006009-M01] - Detection limit for recombinant GST tagged RHEB is approximately 3ng/ml as a capture antibody.
