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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX101983 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101983, RRID:AB_10617907
- Product name
- RAP1 antibody [N2C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE GTX101983 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) B: HCT116 10% SDS PAGE RAP1 antibody GTX101983 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAP1 antibody [N2C3] detects RAP1 protein at nucleus and cytoplasm by immunofluorescent analysis.Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RAP1 protein stained by RAP1 antibody [N2C3] (GTX101983) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RAP1 antibody immunoprecipitates RAP1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 £gg of pre-immune rabbit IgG B : Immunoprecipitation of RAP1 by 3 £gg of RAP1 antibody (GTX101983) 10% SDS-PAGE The immunoprecipitated RAP1 protein was detected by RAP1 antibody (GTX101983) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using RAP1(GTX101983) antibody at 1:500 dilution.
