AP2856c

antibody from Abgent

Targeting: FEN1 FEN-1, MF1, RAD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP2856c

Provider

Abgent

Proper citation

Abgent Cat#AP2856c, RRID:AB_1967656

Product name

FEN1 Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-FEN1 Antibody (Center) (Cat.#AP2856c) in HepG2 cell line lysates (35ug/lane). FEN1(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of FEN1 (arrow) using rabbit polyclonal FEN1 Antibody (Center) (Cat.#AP2856c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FEN1 gene.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Immunofluorescent analysis of U251 cells, using FEN1 Antibody (Center) (Cat. #AP2856c). AP2856c was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue)."

Primary Ab dilution

1:100