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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
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- Western blot [1]
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- Product number
- NB100-150 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-150, RRID:AB_10002467
- Product name
- Mouse Monoclonal FEN-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This is specific for FEN-1.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Adenomatous polyposis coli interacts with flap endonuclease 1 to block its nuclear entry and function.
Adenomatous polyposis coli interacts with flap endonuclease 1 to block its nuclear entry and function.
Prostate cancer cell lines under hypoxia exhibit greater stem-like properties.
Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair.
Assembly of the base excision repair complex on abasic DNA and role of adenomatous polyposis coli on its functional activity.
Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding.
DNA polymerase beta-dependent long patch base excision repair in living cells.
Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells.
Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Long patch base excision repair in mammalian mitochondrial genomes.
Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways.
Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair.
Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria.
HMGB1 is a cofactor in mammalian base excision repair.
Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways.
Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay.
Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen.
Cell cycle-dependent and DNA damage-inducible nuclear localization of FEN-1 nuclease is consistent with its dual functions in DNA replication and repair.
Jaiswal AS, Armas ML, Izumi T, Strauss PR, Narayan S
Neoplasia (New York, N.Y.) 2012 Jun;14(6):495-508
Neoplasia (New York, N.Y.) 2012 Jun;14(6):495-508
Adenomatous polyposis coli interacts with flap endonuclease 1 to block its nuclear entry and function.
Jaiswal AS, Armas ML, Izumi T, Strauss PR, Narayan S
Neoplasia (New York, N.Y.) 2012 Jun;14(6):495-508
Neoplasia (New York, N.Y.) 2012 Jun;14(6):495-508
Prostate cancer cell lines under hypoxia exhibit greater stem-like properties.
Ma Y, Liang D, Liu J, Axcrona K, Kvalheim G, Stokke T, Nesland JM, Suo Z
PloS one 2011;6(12):e29170
PloS one 2011;6(12):e29170
Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair.
Tann AW, Boldogh I, Meiss G, Qian W, Van Houten B, Mitra S, Szczesny B
The Journal of biological chemistry 2011 Sep 16;286(37):31975-83
The Journal of biological chemistry 2011 Sep 16;286(37):31975-83
Assembly of the base excision repair complex on abasic DNA and role of adenomatous polyposis coli on its functional activity.
Jaiswal AS, Narayan S
Biochemistry 2011 Mar 22;50(11):1901-9
Biochemistry 2011 Mar 22;50(11):1901-9
Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding.
Guo Z, Zheng L, Xu H, Dai H, Zhou M, Pascua MR, Chen QM, Shen B
Nature chemical biology 2010 Oct;6(10):766-73
Nature chemical biology 2010 Oct;6(10):766-73
DNA polymerase beta-dependent long patch base excision repair in living cells.
Asagoshi K, Liu Y, Masaoka A, Lan L, Prasad R, Horton JK, Brown AR, Wang XH, Bdour HM, Sobol RW, Taylor JS, Yasui A, Wilson SH
DNA repair 2010 Feb 4;9(2):109-19
DNA repair 2010 Feb 4;9(2):109-19
Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells.
Kong X, Mohanty SK, Stephens J, Heale JT, Gomez-Godinez V, Shi LZ, Kim JS, Yokomori K, Berns MW
Nucleic acids research 2009 May;37(9):e68
Nucleic acids research 2009 May;37(9):e68
Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.
Guo Z, Zheng L, Dai H, Zhou M, Xu H, Shen B
Nucleic acids research 2009 Jun;37(10):3431-41
Nucleic acids research 2009 Jun;37(10):3431-41
Long patch base excision repair in mammalian mitochondrial genomes.
Szczesny B, Tann AW, Longley MJ, Copeland WC, Mitra S
The Journal of biological chemistry 2008 Sep 26;283(39):26349-56
The Journal of biological chemistry 2008 Sep 26;283(39):26349-56
Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways.
Guo Z, Chavez V, Singh P, Finger LD, Hang H, Hegde ML, Shen B
Journal of molecular biology 2008 Mar 28;377(3):679-90
Journal of molecular biology 2008 Mar 28;377(3):679-90
Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair.
Guo Z, Qian L, Liu R, Dai H, Zhou M, Zheng L, Shen B
Molecular and cellular biology 2008 Jul;28(13):4310-9
Molecular and cellular biology 2008 Jul;28(13):4310-9
Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria.
Liu P, Qian L, Sung JS, de Souza-Pinto NC, Zheng L, Bogenhagen DF, Bohr VA, Wilson DM 3rd, Shen B, Demple B
Molecular and cellular biology 2008 Aug;28(16):4975-87
Molecular and cellular biology 2008 Aug;28(16):4975-87
HMGB1 is a cofactor in mammalian base excision repair.
Prasad R, Liu Y, Deterding LJ, Poltoratsky VP, Kedar PS, Horton JK, Kanno S, Asagoshi K, Hou EW, Khodyreva SN, Lavrik OI, Tomer KB, Yasui A, Wilson SH
Molecular cell 2007 Sep 7;27(5):829-41
Molecular cell 2007 Sep 7;27(5):829-41
Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways.
Kundu CN, Balusu R, Jaiswal AS, Narayan S
Carcinogenesis 2007 Oct;28(10):2089-95
Carcinogenesis 2007 Oct;28(10):2089-95
Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay.
Shibata Y, Nakamura T
The Journal of biological chemistry 2002 Jan 4;277(1):746-54
The Journal of biological chemistry 2002 Jan 4;277(1):746-54
Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen.
Kedar PS, Kim SJ, Robertson A, Hou E, Prasad R, Horton JK, Wilson SH
The Journal of biological chemistry 2002 Aug 23;277(34):31115-23
The Journal of biological chemistry 2002 Aug 23;277(34):31115-23
Cell cycle-dependent and DNA damage-inducible nuclear localization of FEN-1 nuclease is consistent with its dual functions in DNA replication and repair.
Qiu J, Li X, Frank G, Shen B
The Journal of biological chemistry 2001 Feb 16;276(7):4901-8
The Journal of biological chemistry 2001 Feb 16;276(7):4901-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FEN-1 Antibody (4E7) [NB100-150] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500.
