Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-60424 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60424, RRID:AB_905378
- Product name
- Rabbit Polyclonal XPV/DNA polymerase eta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references c-Jun N-terminal kinase-mediated Rad18 phosphorylation facilitates Polη recruitment to stalled replication forks.
Phosphorylated Rad18 directs DNA polymerase η to sites of stalled replication.
Barkley LR, Palle K, Durando M, Day TA, Gurkar A, Kakusho N, Li J, Masai H, Vaziri C
Molecular biology of the cell 2012 May;23(10):1943-54
Molecular biology of the cell 2012 May;23(10):1943-54
Phosphorylated Rad18 directs DNA polymerase η to sites of stalled replication.
Day TA, Palle K, Barkley LR, Kakusho N, Zou Y, Tateishi S, Verreault A, Masai H, Vaziri C
The Journal of cell biology 2010 Nov 29;191(5):953-66
The Journal of cell biology 2010 Nov 29;191(5):953-66
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of Human PolH on HeLa whole cell lysate using NB100-60424. PolH was also immunoprecipitated by rabbit anti-PolH antibody NB100-60423.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiPolH antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of human PolH by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PolH antibody NB100-60424 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of human XPV/DNA polymerase eta/PolH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-PolH antibody NB100-60424 (lot 3) used for IP at 6 ug per reaction. PolH was also immunoprecipitated by a previous lot of this antibody (lot 2) and rabbit anti-PolH antibody NB100-60423. For blotting immunoprecipitated PolH, NB100-60424 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 minutes.
