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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- GTX113849 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113849, RRID:AB_2038100
- Product name
- TBX5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TBX5 antibody detects TBX5 protein by western blot analysis.A. 50 ?g rat brain lysate/extract 7.5% SDS-PAGETBX5 antibody (GTX113849) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE GTX113849 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HeLa whole cell and nuclear extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with TBX5 antibody (GTX124336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TBX5(GTX113849) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TBX5 antibody detects TBX5 protein at nucleus on rat ovary by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. TBX5 antibody (GTX113849) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TBX5 antibody detects TBX5 protein at nucleus on rat thyroid gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thyroid gland. TBX5 antibody (GTX113849) diluted at 1:500.
