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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [5]
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Validation data
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- Product number
- LS-C185778 - Provider product page
- Provider
- LSBio
- Product name
- FLII / FLI Antibody (aa435-778) LS-C185778
- Antibody type
- Polyclonal
- Description
- Immunoaffinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (30 ug whole cell lysate). A: Raji . 5% SDS PAGE. FLII / FLI antibody diluted at 1:1000
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate). A: Mouse brain. 5% SDS PAGE. FLII / FLI antibody diluted at 1:1000.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using Flightless I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed mouse ESC D3 using Flightless I antibody at 1:200 dilution.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunofluorescence of paraformaldehyde-fixed mouse ESC D3 using Flightless I antibody at 1:200 dilution.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using Flightless I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using Flightless I antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
