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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-95374 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACVR2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Human ACVR2A shares 100% and 98.9% amino acid (aa) sequence identity with mouse and rat ACVR2A, respectively.
- Concentration
- 500 µg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACVR2A in rat kidney extract (lane 1) and HeLa whole cell lysates (lane 2). Samples were incubated with ACVR2A polyclonal antibody (Product # PA5-95374) using a 0.5 µg/mL dilution. developed was performed using enhanced chemiluminescence (ECL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ACVR2A using anti-ACVR2A antibody (Product # PA5-95374). ACVR2A was detected in a section of NRK cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-ACVR2A antibody (Product # PA5-95374)overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ACVR2A in paraffin-embedded human intestinal cancer tissues. Samples were incubated with ACVR2A polyclonal antibody (Product # PA5-95374) at a 1 µg/mL dilution, and developed with Strepavidin-Biotin-Complex.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of ACVR2A in HEPA1-6 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with ACVR2A Polyclonal Antibody (Product # PA5-95374) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of ACVR2A in RH-35 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with ACVR2A Polyclonal Antibody (Product # PA5-95374) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
