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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00007516-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007516-M01, RRID:AB_1137074
- Product name
- XRCC2 monoclonal antibody (M01), clone 2H4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant XRCC2.
- Antigen sequence
MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADE
DSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKS
EGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSE
EIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSH
PSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRK
CSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPS
HASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQD
DSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC- Isotype
- IgG
- Antibody clone number
- 2H4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Dysfunctional homologous recombination mediates genomic instability and progression in myeloma.
Shammas MA, Shmookler Reis RJ, Koley H, Batchu RB, Li C, Munshi NC
Blood 2009 Mar 5;113(10):2290-7
Blood 2009 Mar 5;113(10):2290-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of XRCC2 expression in transfected 293T cell line by XRCC2 monoclonal antibody (M01), clone 2H4.Lane 1: XRCC2 transfected lysate(32 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged XRCC2 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to XRCC2 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
