PA5-19240

antibody from Invitrogen Antibodies

Targeting: TIAL1 TIAR

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19240

Provider

Invitrogen Antibodies

Product name

TIA1/TIAL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine and mouse based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot staining of K562 cell lysate using Product # PA5-19240 at a concentration of 2 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of TIA1 TIAL1 in paraffin embedded human skin using a TIA1 TIAL1 polyclonal antibody (Product #PA5-19240) at a concentration of 3.8 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIG 5 SARS-CoV-2 NP suppresses G3BP-mediated SG formation. (A) A549 cells were treated with 500 muM sodium arsenite (SA) for 1 h to induce SG assembly, and saline was used as a control. G3BP1 (green) and G3BP2 (red) were labeled to visualize SGs by immunofluorescence staining. The nuclei were stained with DAPI (blue). (B) A549 cells expressing GFP-tagged NP SARS-CoV-2 or GFP-vector were treated with 500 muM SA for 1 h to induce SG assembly. TIA1 (red) and G3BP1 (violet) were labeled to visualize SGs by immunofluorescence staining. The nuclei were stained with DAPI (blue). (C) The average SG number per cell (>=10) in panel B was calculated using ImageJ software. All quantitative data are shown as the means +- SDs. * * , P < 0.05. (D) A549 cells transfected with or without a G3BP1/G3BP2 plasmid were infected with SARS-CoV-2 for 24 h and treated with or without 500 muM SA during the last hour. NP SARS-CoV-2 (green) and the SG marker G3BP1 (red) were detected via immunofluorescence staining. The nuclei were stained with DAPI (blue). (E) The average SG number per cell (>=10) in panel D was calculated using ImageJ software. All quantitative data are shown as the means +- SDs. * * , P < 0.05.