Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PB9883 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-RAB7/RAB7A Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for RAB7/RAB7A detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB, ICC/IF, FCM. Reactive species: Human;Mouse;Rat. RAB7/RAB7A information: Molecular Weight: 23490 MW; Subcellular Localization: Cytoplasmic vesicle, phagosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Late endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Lysosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Melanosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasmic vesicle, autophagosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Lipid droplet . Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium (PubMed:21255211). Lipid droplet localization is increased upon ADRB2 stimulation (By similarity); Tissue Specificity: Widely expressed; high expression found in skeletal muscle.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
No comments: Submit comment
Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of RAB7 expression in rat brain extract (lane 1), mouse spleen extract (lane 2), HELA whole cell lysates (lane 3) and MCF-7 whole cell lysates (lane 4). RAB7 at 23KD was detected using rabbit anti- RAB7 Antigen Affinity purified polyclonal antibody (Catalog # PB9883) at0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
- Additional image