NBP2-27398

antibody from Novus Biologicals

Targeting: BAG3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-27398

Provider

Novus Biologicals

Product name

Rabbit Polyclonal BAG3 Antibody

Antibody type

Polyclonal

Description

Unpurified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.05 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Chaperone Mediated Autophagy Degrades TDP-43 Protein and Is Affected by TDP-43 Aggregation.

Ormeño F, Hormazabal J, Moreno J, Riquelme F, Rios J, Criollo A, Albornoz A, Alfaro IE, Budini M
Frontiers in molecular neuroscience 2020;13:19

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Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.

Li X, Lu J, Kan Q, Li X, Fan Q, Li Y, Huang R, Slipicevic A, Dong HP, Eide L, Wang J, Zhang H, Berge V, Goscinski MA, Kvalheim G, Nesland JM, Suo Z
Scientific reports 2017 Jul 11;7(1):5081

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Expression of Bcl-2 and the antiapoptotic BAG family proteins in ovarian cancer.

Aust S, Pils S, Polterauer S, Horvat R, Cacsire Castillo-Tong D, Pils D, Dudek G, Schmid B, Speiser P, Reinthaller A, Grimm C
Applied immunohistochemistry & molecular morphology : AIMM 2013 Dec;21(6):518-24

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Bag3 promotes resistance to apoptosis through Bcl-2 family members in non-small cell lung cancer.

Zhang Y, Wang JH, Lu Q, Wang YJ
Oncology reports 2012 Jan;27(1):109-13

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Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis.

Goldman A, Chen HD, Roesly HB, Hill KA, Tome ME, Dvorak B, Bernstein H, Dvorak K
American journal of physiology. Gastrointestinal and liver physiology 2011 Feb;300(2):G292-302

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The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.

Liao Q, Ozawa F, Friess H, Zimmermann A, Takayama S, Reed JC, Kleeff J, Büchler MW
FEBS letters 2001 Aug 17;503(2-3):151-7

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The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.

Liao Q, Ozawa F, Friess H, Zimmermann A, Takayama S, Reed JC, Kleeff J, Büchler MW
FEBS letters 2001 Aug 17;503(2-3):151-7

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BAG3 Antibody [NBP2-27398] - Analysis of BAG3 using NBP2-27398 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: BAG3 Antibody [NBP2-27398] - Simple Western lane view shows a specific band for BAG3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398] - Frozen mouse muscle tissue section stained for BAG-3 expression using NBP2-27398 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-27398] - Staining of human pancreas tissue sections using this antibody at 1:2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.