Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-27398 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal BAG3 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Chaperone Mediated Autophagy Degrades TDP-43 Protein and Is Affected by TDP-43 Aggregation.
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.
Expression of Bcl-2 and the antiapoptotic BAG family proteins in ovarian cancer.
Bag3 promotes resistance to apoptosis through Bcl-2 family members in non-small cell lung cancer.
Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis.
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.
Ormeño F, Hormazabal J, Moreno J, Riquelme F, Rios J, Criollo A, Albornoz A, Alfaro IE, Budini M
Frontiers in molecular neuroscience 2020;13:19
Frontiers in molecular neuroscience 2020;13:19
Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells.
Li X, Lu J, Kan Q, Li X, Fan Q, Li Y, Huang R, Slipicevic A, Dong HP, Eide L, Wang J, Zhang H, Berge V, Goscinski MA, Kvalheim G, Nesland JM, Suo Z
Scientific reports 2017 Jul 11;7(1):5081
Scientific reports 2017 Jul 11;7(1):5081
Expression of Bcl-2 and the antiapoptotic BAG family proteins in ovarian cancer.
Aust S, Pils S, Polterauer S, Horvat R, Cacsire Castillo-Tong D, Pils D, Dudek G, Schmid B, Speiser P, Reinthaller A, Grimm C
Applied immunohistochemistry & molecular morphology : AIMM 2013 Dec;21(6):518-24
Applied immunohistochemistry & molecular morphology : AIMM 2013 Dec;21(6):518-24
Bag3 promotes resistance to apoptosis through Bcl-2 family members in non-small cell lung cancer.
Zhang Y, Wang JH, Lu Q, Wang YJ
Oncology reports 2012 Jan;27(1):109-13
Oncology reports 2012 Jan;27(1):109-13
Characterization of squamous esophageal cells resistant to bile acids at acidic pH: implication for Barrett's esophagus pathogenesis.
Goldman A, Chen HD, Roesly HB, Hill KA, Tome ME, Dvorak B, Bernstein H, Dvorak K
American journal of physiology. Gastrointestinal and liver physiology 2011 Feb;300(2):G292-302
American journal of physiology. Gastrointestinal and liver physiology 2011 Feb;300(2):G292-302
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.
Liao Q, Ozawa F, Friess H, Zimmermann A, Takayama S, Reed JC, Kleeff J, Büchler MW
FEBS letters 2001 Aug 17;503(2-3):151-7
FEBS letters 2001 Aug 17;503(2-3):151-7
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.
Liao Q, Ozawa F, Friess H, Zimmermann A, Takayama S, Reed JC, Kleeff J, Büchler MW
FEBS letters 2001 Aug 17;503(2-3):151-7
FEBS letters 2001 Aug 17;503(2-3):151-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BAG3 Antibody [NBP2-27398] - Analysis of BAG3 using NBP2-27398 at 1:2000. Tissue lysates, normalized for total protein (20 ug/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: BAG3 Antibody [NBP2-27398] - Simple Western lane view shows a specific band for BAG3 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: BAG3 Antibody [NBP2-27398] - Frozen mouse muscle tissue section stained for BAG-3 expression using NBP2-27398 at 1:2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-27398] - Staining of human pancreas tissue sections using this antibody at 1:2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.