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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP21600SU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21600SU-S, RRID:AB_10758451
- Product name
- anti BAG3
- Antibody type
- Polyclonal
- Antigen
- A recombinant protein fragment corresponding to the C-terminal 196 amino acids of human BAG-3.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 50 µl
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of BAG-3 using AP21600SU-S at 1/2000. Tissue lysates, normalized for total protein (20 µg/lane), were from a 4 week old male mouse. BAG-3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections using AP21600SU-S at 1/2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Frozen mouse muscle tissue section stained for BAG-3 expression using AP21600SU-S at 1/2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG-3 localizes with Z-disk proteins.
