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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- PAB0330 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0330, RRID:AB_1019075
- Product name
- BAG3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant BAG3.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Modulation of BAG3 Expression and Proteasomal Activity by sAPPα Does Not Require Membrane-Tethered Holo-APP.
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.
Kundu A, Milosch N, Antonietti P, Baumkötter F, Zymny A, Müller UC, Kins S, Hajieva P, Behl C, Kögel D
Molecular neurobiology 2015 Nov 2;
Molecular neurobiology 2015 Nov 2;
The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines.
Liao Q, Ozawa F, Friess H, Zimmermann A, Takayama S, Reed JC, Kleeff J, Büchler MW
FEBS letters 2001 Aug 17;503(2-3):151-7
FEBS letters 2001 Aug 17;503(2-3):151-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BAG3. Using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. Tissue lysates, normalized for total protein (20 ug/lane) , were from a 4 week old male mouse. BAG3 expression was detected at highest levels in skeletal (quadriceps and diaphragm) and smooth (heart) muscle specimens.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin-embedded human pancreas tissue sections. Using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. A, normal pancreas. B and C, pancreatic cancer. Hematoxylin-eosin counterstain.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Frozen mouse muscle tissue section stained for BAG3 expression using BAG3 polyclonal antibody (Cat # PAB0330) at 1 : 2000. The tissue section was fixed in 3.8% paraformaldehyde prior to staining. BAG3 localizes with Z-disk proteins.
- Validation comment
- Immunohistochemistry (Frozen sections)
