Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 10599-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BAG3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SREGSPARS STPLHSPSPI RVHTVVDRPQ QPMTHRETAP VSQPENKPES KPGPVGPELP PGHIPIQVIR KEVDSKPVSQ KPPPPSEKVE VKVPPAPVPC PPPSPGPSAV PSSPKSVATE ERAAPSTAPA EATPPKPGEA EAPPKHPGVL KVEAILEKVQ GLEQAVDNFE GKKTDKKYLM IEEYLTKELL ALDSVDPEGR ADVRQARRDG VRKVQTILEK LEQKAIDVPG QVQVYELQPS NLEADQPLQA IMEMGAVAAD KGKKNAGNAE DPHTETQQPE ATAAATSNPS SMTDTPGNPA AP (275-575 aa encoded by BC006418)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.48 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse uterus tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DU 145 cells were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 10599-1-AP (BAG3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using (Product # 10599-1-AP) (BAG3 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using BAG3 antibody 10599-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10599-1-AP ( BAG3 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 10599-1-AP ( BAG3 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-BAG3 (IP:10599-1-AP, 4ug; Detection:10599-1-AP 1:1000) with K-562 cells lysate 11000ug.