ALX-803-323-C100

antibody from Enzo Life Sciences

Targeting: BAG3

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ALX-803-323-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-803-323-C100, RRID:AB_2050793

Product name

BAG-3 monoclonal antibody (AC-1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

AC-1

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.

References

Submitted references

Cordyceps sinensis attenuates renal fibrosis and suppresses BAG3 induction in obstructed rat kidney.

Du F, Li S, Wang T, Zhang HY, Zong ZH, Du ZX, Li DT, Wang HQ, Liu B, Miao JN, Bian XH
American journal of translational research 2015;7(5):932-40

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HSPB8 and the Cochaperone BAG3 Are Highly Expressed During the Synthetic Phase of Rat Myometrium Programming During Pregnancy.

Marsh NM, Wareham A, White BG, Miskiewicz EI, Landry J, MacPhee DJ
Biology of reproduction 2015 May;92(5):131

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Expression of the anti-apoptotic protein BAG3 in human melanomas.

Franco R, Scognamiglio G, Salerno V, Sebastiani A, Cennamo G, Ascierto PA, Botti G, Turco MC, Rosati A
The Journal of investigative dermatology 2012 Jan;132(1):252-4

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BAG3 protein delocalisation in prostate carcinoma.

Staibano S, Mascolo M, Di Benedetto M, Vecchione ML, Ilardi G, Di Lorenzo G, Autorino R, Salerno V, Morena A, Rocco A, Turco MC, Morelli E
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2010 Oct;31(5):461-9

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Evidence for modulation of BAG3 by polyomavirus JC early protein.

Basile A, Darbinian N, Kaminski R, White MK, Gentilella A, Turco MC, Khalili K
The Journal of general virology 2009 Jul;90(Pt 7):1629-40

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Identification of a synaptosome-associated form of BAG3 protein.

Bruno AP, Festa M, Dal Piaz F, Rosati A, Turco MC, Giuditta A, Marzullo L
Cell cycle (Georgetown, Tex.) 2008 Oct;7(19):3104-5

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Bag3 gene expression is regulated by heat shock factor 1.

Franceschelli S, Rosati A, Lerose R, De Nicola S, Turco MC, Pascale M
Journal of cellular physiology 2008 Jun;215(3):575-7

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Activation of BAG3 by Egr-1 in response to FGF-2 in neuroblastoma cells.

Gentilella A, Passiatore G, Deshmane S, Turco MC, Khalili K
Oncogene 2008 Aug 28;27(37):5011-8

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of BAG-3 expressionÊusing MAb to BAG-3 (AC-1) (Prod. No. ALX-803-323) at a concentration of 0.3µg/ml.A) NPA cellsB) HeLa cellsC) HUVEC cells

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry of human primary breast adenocarcinoma with MAb to BAG-3 (AC-1) (Prod. No. ALX-803-323).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunoprecipitation of BAG-3 from HUVEC cell extract using MAb to BAG-3 (AC-1) (Prod. No. ALX-803-323).A) 3µg MAb to BAG-3 (AC-1), 300µg HUVEC cell extractB) 3µg mouse IgG, 300µg HUVEC cell extractC) 30µg HUVEC cell extract

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Indirect immunofluorescence of BAG-3 from NPA (human thyroid carcinoma) cells using MAb to BAG-3 (AC-1) (Prod. No. ALX-803-323)Êand secondary antibody rhodamine-conjugated anti-mouse IgG.A) Negative control; Mouse IgG at 3µg/mlB) MAb to BAG-3 (AC-1) at 3µg/ml