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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX113936 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113936, RRID:AB_2036564
- Product name
- Calcium binding protein p22 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references CHP1 Regulates Compartmentalized Glycerolipid Synthesis by Activating GPAT4.
Zhu XG, Nicholson Puthenveedu S, Shen Y, La K, Ozlu C, Wang T, Klompstra D, Gultekin Y, Chi J, Fidelin J, Peng T, Molina H, Hang HC, Min W, Birsoy K
Molecular cell 2019 Apr 4;74(1):45-58.e7
Molecular cell 2019 Apr 4;74(1):45-58.e7
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calcium binding protein P22 antibody detects CHP protein by western blot analysis.A. 50 ?g mouse liver lysate/extract 12% SDS-PAGECalcium binding protein P22 antibody (GTX113936) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calcium binding protein P22 antibody detects CHP protein by western blot analysis.A. 50 ?g rat liver lysate/extract 12% SDS-PAGECalcium binding protein P22 antibody (GTX113936) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa B: HepG2 (GTX27900) 12% SDS PAGE GTX113936 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody (GTX113936) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calcium binding protein P22(GTX113936) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Calcium binding protein P22 antibody (GTX113936) dilution: 1:500.
