Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-97706 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EN2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3.5 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EN2 using a EN2 Polyclonal antibody (Product # PA5-97706) at a concentration of 2.8 µg/mL. Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 35 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EN2 using a EN2 Polyclonal antibody (Product # PA5-97706) at a concentration of 2.8 µg/mL. Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 35 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EN2 in K562 whole cell lysate, and MCF-7 whole cell lysate using a EN2 polyclonal antibody (Product # PA5-97706) at a concentration of 2.8 µg/mL. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EN2 in Hela cells using a EN2 polyclonal antibody (Product # PA5-97706) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of EN2 in paraffin embedded human liver cancer using a EN2 polyclonal antibody (Product # PA5-97706) at a dilution of 1:100.