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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- GTX107701 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107701, RRID:AB_1951901
- Product name
- SHANK1 antibody [C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE GTX107701 diluted at 1:1000
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- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SHANK1 antibody [C3], C-term detects SHANK1 protein at cytoplasm by immunofluorescent analysis.Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.Blue: Hoechst 33342 staining.
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- GeneTex (provider)
- Main image
- Experimental details
- SHANK1 antibody [C3], C-term detects SHANK1 protein at cell body and synaptic vesicles by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SHANK1(GTX107701) antibody at 1:500 dilution.
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- GeneTex (provider)
- Main image
- Experimental details
- SHANK1 antibody [C3] detects SHANK1 protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: SHANK1 antibody [C3] (GTX107701) diluted at 1:250. Blue: DAPI
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- GeneTex (provider)
- Main image
- Experimental details
- SHANK1 antibody [C3], C-term detects SHANK1 Protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse cerebellum. Green: SHANK1 stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SHANK1 antibody [C3], C-term detects SHANK1 protein by immunohistochemical analysis.Samples: Paraffin-Embedded mouse retina.Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
